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-acetyl by-product. This result's consistent with the formation or collapse from the tetrahedral intermediate as the rate limiting phase from the catalytic mechanism of NagA.

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The result of metal substitution around the catalytic constants for NagA is often used To judge the contributions produced by these divalent cations on enzymatic exercise (22, 23). The relationship on the value of k

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You'll find an important amount of issues concerning the system for substrate hydrolysis by NagA that happen to be unresolved. It really is unclear how the divalent cation binds throughout the active site of NagA And just how the drinking water molecule is activated for nucleophilic attack over the amide bond. The disparity while in the metallic ligation techniques between the E. coli

Incubation of the apo-enzyme with 1 equal of Zn2+ for half-hour thoroughly restored catalytic activity. The titration of apo-enzyme daftar naga26 with various amounts of ZnCl2 is revealed in Figure 1. These outcomes reveal that NagA from E. coli

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